As Tina begins to introduce the first tale of terror, strange occurrences start to happen around her. Equipment starts to malfunction, and dark figures begin to appear in the shadows. Tina starts to feel a sense of dread creeping over her, but she tries to brush it off as just her imagination.
The first story is about a group of college students who visit an abandoned asylum to explore its haunted history. However, they quickly discover that the asylum is not as abandoned as they thought and they become trapped inside, hunted by the vengeful spirits of the former patients.
As Tina moves on to the second story, she realizes that something is very wrong. She tries to leave the lab, but the doors and windows are all sealed shut. The second story is about a young couple who moves into a new house, only to discover that it is haunted by the restless spirits of the previous occupants. As they try to uncover the truth about the house's dark history, they become entangled in a web of supernatural horror.
Tina becomes increasingly desperate as the night wears on, but there seems to be no escape from the shadowy presence that stalks her. The third story is about a group of friends who go on a camping trip in the woods, only to stumble upon a mysterious portal to another dimension. As they explore this strange new world, they discover that it is ruled by an ancient and malevolent force that seeks to keep them trapped there forever.
In the fourth story, Tina finally confronts the shadowy menace that has been haunting her all night. It is revealed to be a vengeful ghost who was once a lab technician like her. The ghost is angry at the way she was mistreated by her colleagues and seeks revenge on all those who work in the lab.
The fifth and final story is about Tina herself, as she tries to survive the ghost's wrath. The ghost possesses the lab equipment and turns it against her, forcing her to fight for her life. In the end, Tina manages to defeat the ghost and escape the lab, but not before experiencing the true terror of the witching hour.