The scientific expedition in the movie is led by Dr. David Reed, who is joined by Dr. Mark Williams and his fiancée Kay Lawrence. They travel deep into the Amazon rainforest to search for evidence of prehistoric life. Along the way, they encounter the Gill-Man, a prehistoric humanoid creature with gills and scales. The creature becomes obsessed with Kay and begins to stalk the members of the expedition.
After the Gill-Man is captured, it manages to break free and kidnap Kay. Dr. Reed and Dr. Williams form a rescue party to save her, but they are constantly hindered by the creature's cunning and strength. Meanwhile, the Gill-Man becomes more and more possessive of Kay, leading to a thrilling showdown between the two groups.
The film was shot in 3D to fully showcase the Gill-Man's underwater world and eerie presence. The film's success spawned two sequels, Revenge of the Creature and The Creature Walks Among Us. The Creature from the Black Lagoon is now considered a classic of the horror genre and an iconic creature feature.