In Bring It On: Cheer or Die, the cheerleading squad of Great Oaks High School is in a tough spot. Due to a recent scandal involving hazing, the school has implemented strict rules surrounding their practices. Determined to win at regionals, the cheerleaders sneak into an abandoned and creepy closed school gym to practice.
However, their practice sessions take a deadly turn when members of the squad start to disappear one by one. The remaining cheerleaders become increasingly paranoid and suspect each other of foul play. As they dig deeper into the mystery, they uncover a sinister plot that involves a former cheerleader seeking revenge against the school for past transgressions.
The squad races against time to figure out who the killer is, all while maintaining their focus for regionals. Along the way, they encounter several obstacles and twists that challenge their unity and individual strengths. In the end, the cheerleaders come together to defeat the killer and triumph at regionals, proving that nothing can bring them down.